Open AccessFlavobacterium laiguense sp. nov., a psychrophilic bacterium isolated from Laigu glacier on the Tibetan Plateau
Author(s) -
Leilei Yang,
Qing Liu,
HongCan Liu,
YuGuang Zhou,
YuHua Xin
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003400
Subject(s) - 16s ribosomal rna , biology , flavobacterium , psychrophile , phylogenetic tree , strain (injury) , bacteria , microbiology and biotechnology , flavobacteriaceae , genomic dna , genus , phylogenetics , botany , gene , bacteroidetes , genetics , pseudomonas , anatomy
One bacterial strain, denoted as LB2P30 T , was isolated from Laigu glacier located on the Tibetan Plateau, PR China. Strain LB2P30 T was an aerobic, non-motile, rod-shaped, yellow-pigmented and Gram-stain-negative bacterium. The temperature range for growth was 4-20 °C (optimum, 14 °C). The phylogenetic analysis based on partial 16S rRNA gene sequences showed that strain LB2P30 T belonged to the genus Flavobacterium and was most similar to Flavobacterium fluvii (98.12 %) and Flavobacterium limicola (97.91 %). The average nucleotide identity values between strain LB2P30 T and its closest relatives, F. fluvii DSM 19978 T and F. limicola DSM 15094 T , were 76.73 % and 77.40 %, respectively. Cells of strain LB2P30 T contained summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c) and iso-C15 : 0 as the predominant fatty acids and menaquinone-6 as the sole menaquinone. The genomic DNA G+C content was 34.74 mol%. Based on the phenotypic, chemotaxonomic, genotypic and phylogenetic characteristics, we propose strain LB2P30 T as a novel species of the genus Flavobacterium with the nomenclature of Flavobacteriumlaiguense sp. nov. The type strain is LB2P30 T (=CGMCC 1.11271 T =NBRC 113059 T ).