Open AccessFlavobacterium piscinae sp. nov., isolated from a fish pond
Author(s) -
Wen-Ming Chen,
Yaping Guo,
Soon-Wo Kwon,
Shih-Yi Sheu
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003391
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , genomic dna , strain (injury) , flavobacterium , gene , microbiology and biotechnology , phylogenetics , bacteria , genetics , anatomy , pseudomonas
Strain ICH-30 T was isolated from a freshwater fish pond in Taiwan. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 92 protein clusters indicated that strain ICH-30 T formed a phylogenetic lineage in the genus Flavobacterium. Strain ICH-30 T was most closely related to Flavobacteriumlacus NP180 T with 97.0 % 16S rRNA gene sequence similarity. Strain ICH-30 T showed 70.0-83.1 % average nucleotide identity and 16.5-23.7 % digital DNA-DNA hybridization identity with the type strains of other closely related Flavobacterium species. Cells of strain ICH-30 T were Gram-stain-negative, strictly aerobic, non-motile, rod-shaped and formed dark orange colonies. Optimal growth occurred at 20-30 °C, pH 8-9 and 0-0.5 % NaCl. Strain ICH-30 T contained iso-C15 : 1 G and iso-C15 : 0 as the predominant fatty acids. The major hydroxyl fatty acids were iso-C17 : 0 3-OH, iso-C16 : 0 3-OH and iso-C15 : 0 3-OH. The polar lipid profile consisted of phosphatidylethanolamine, six uncharacterized aminophospholipids, one uncharacterized phospholipid and two uncharacterized lipids. The major polyamine was homospermidine. The only isoprenoid quinone was MK-6. The DNA G+C content of the genomic DNA was 34.3 mol%. On the basis of the phylogenetic inference and phenotypic data, strain ICH-30 T should be classified as a novel species, for which the name Flavobacterium piscinae sp. nov. is proposed. The type strain is ICH-30 T (=BCRC 81122 T =LMG 30579 T =KCTC 62513 T ).