Open AccessPectobacterium polonicum sp. nov. isolated from vegetable fields
Author(s) -
Małgorzata Waleron,
Agnieszka Misztak,
Joanna Jońca,
Magda Furmaniak,
Krzysztof Waleron
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003387
Subject(s) - pectobacterium , biology , housekeeping gene , 16s ribosomal rna , microbiology and biotechnology , botany , multilocus sequence typing , bacteria , genetics , gene , genotype , gene expression
Gram-stain-negative, rod-shaped pectinolytic bacteria strains designated as DPMP315 T , DPMP316, DPMP317 and DPMP318 isolated from groundwater sampled from a vegetable field in the North of Poland, were subjected to the polyphasic analyses. Multilocus sequence analyses based on five housekeeping genes (gyrA, recA, recN, rpoA and rpoS) revealed their distinctiveness from the other species of the genus, simultaneously indicating that the newly described species, Pectobacterium punjabense, as well as Pectobacterium parmentieri and P. wasabiae, to be the closest relatives. In silico DNA-DNA hybridization (<43.1 %) and average nucleotide identity (<92.5 %) values of strain DPMP315 T with other type strains of species of the genus Pectobacterium supported the delineation of the novel strain as representing a novel species. The phenotypic comparisons, fatty acid methyl esters compositions, genetic rep PCR fingerprint and detailed whole-cell MALDI-TOF mass spectrometry proteomic profiles permitted the differentiation of Polish strains from the type strains of all other known species of the genus Pectobacterium. The results of polyphasic analyses performed for four Polish strains are the basis for the distinction of the novel species. Here, we propose to establish DPMP315 T as a type strain (=PCM3006 T =LMG 31077 T ) with the name Pectobacterium polonicum sp. nov.