Open AccessSiculibacillus lacustris gen. nov., sp. nov., a new rosette-forming bacterium isolated from a freshwater crater lake (Lake St. Ana, Romania)
Author(s) -
Tamás Felföldi,
Zsuzsanna Márton,
Attila Szabó,
Anikó Mentes,
Károly Bóka,
Károly Màrialigeti,
István Máthé,
Mihály Koncz,
Peter Schümann,
Erika Tóth
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003385
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phosphatidylglycerol , microbiology and biotechnology , botany , genus , ribosomal rna , bacteria , lineage (genetic) , genomic dna , gene , genetics , anatomy , phosphatidylcholine , phospholipid , membrane
A new aerobic alphaproteobacterium, strain SA-279 T , was isolated from a water sample of a crater lake. The 16S rRNA gene sequence analysis revealed that strain SA-279 T formed a distinct lineage within the family Ancalomicrobiaceae and shared the highest pairwise similarity values with Pinisolibacterravus E9 T (96.4 %) and Ancalomicrobiumadetum NBRC 102456 T (94.2 %). Cells of strain SA-279 T were rod-shaped, motile, oxidase and catalase positive, and capable of forming rosettes. Its predominant fatty acids were C18 : 1ω7c (69.0 %) and C16 : 1ω7c (22.7 %), the major respiratory quinone was Q-10, and the main polar lipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylcholine, phosphatidylglycerol, an unidentified aminophospholipid and an unidentified lipid. The G+C content of the genomic DNA of strain SA-279 T was 69.2 mol%. On the basis of the phenotypic, chemotaxonomic and molecular data, strain SA-279 T is considered to represent a new genus and species within the family Ancalomicrobiaceae, for which the name Siculibacillus lacustris gen. nov., sp. nov. is proposed. The type strain is SA-279 T (=DSM 29840 T =JCM 31761 T ).