Open AccessDeinococcus fonticola sp. nov., isolated from a radioactive thermal spring in Hungary
Author(s) -
Judit Makk,
Nóra Tünde Enyedi,
Erika Tóth,
Dóra Anda,
Attila Szabó,
Tamás Felföldi,
Peter Schümann,
Judit Mádl-Szőnyi,
Andrea K. Borsodi
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003383
Subject(s) - deinococcus , biology , 16s ribosomal rna , peptidoglycan , strain (injury) , microbiology and biotechnology , glycolipid , ribosomal rna , bacteria , cell wall , botany , gene , biochemistry , genetics , deinococcus radiodurans , anatomy
A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium, designated strain FeSDHB5-19 T , was isolated from a biofilm sample collected from a radioactive thermal spring (Budapest, Hungary), after exposure to 5 kGy gamma radiation. A polyphasic approach was used to study the taxonomic properties of strain FeSDHB5-19 T , which had highest 16S rRNA gene sequence similarity to Deinococcus antarcticus G3-6-20 T (96.5 %). The 16S rRNA gene sequence similarity to type strains of other Deinococcus species were 93.0 % or lower. The DNA G+C content of the draft genome sequence, consisting of 3.9 Mb, was 63.9 mol%. Strain FeSHDB5-19 T was found to grow at temperatures of 10-32 °C (optimum, 28 °C) and pH 5-10 (pH 6.5-7.5) and tolerated up to 1.5 % NaCl (w/v) with optimum growth at 0-0.5 % NaCl. The predominant fatty acids (>10 %) were C16 : 0 and C16 : 1ω7c. The cell-wall peptidoglycan type was A3β l-Orn-Gly1-2. The whole-cell sugars were glucose and low amounts of galactose. Strain FeSDHB5-19 T possessed MK-8 as the predominant respiratory quinone, typical of the genus Deinococcus. The polar lipid profile contained unidentified phosphoglycolipids and unidentified glycolipids. The isolate was found to be highly resistant to gamma (D10<8 kGy) and UV (D10~800 J m -2 ) radiation. According to its genotypic, phenotypic and chemotaxonomic characteristics, strain FeSDHB5-19 T represents a novel species in the genus Deinococcus, for which the name Deinococcusfonticola sp. nov. is proposed. The type strain is FeSDHB5-19 T (=NCAIM B.02639 T =DSM 106917 T ).