Open AccessGemmobacter lutimaris sp. nov., a marine bacterium isolated from a tidal flat
Author(s) -
Yeonjae Yoo,
Dong Wan Lee,
Hanbyul Lee,
Bong-Oh Kwon,
Jong Seong Khim,
Un Hyuk Yim,
Hong-Jae Park,
Byeonghyeok Park,
In Geol Choi,
Beom Seok Kim,
Seong Woo Jeon,
Gyu Hyeok Kim,
Jae Jin Kim
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003375
Subject(s) - biology , tidal flat , bacteria , marine bacteriophage , microbiology and biotechnology , zoology , ecology , paleontology , sediment
A novel cream-pigmented marine bacterium, designated strain YJ-T1-11 T , was isolated from a tidal flat at Yeongjong-do, Republic of Korea. Cells were rod-shaped, non-motile, aerobic, Gram-reaction-negative, oxidase-positive and catalase-positive. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain YJ-T1-11 T clustered with Gemmobacter fontiphilus JS43 T (98.3 %) within the genus Gemmobacter and its closest neighbours were G.emmobacter aquatilis DSM 3857 T (98.5 %), Gemmobacter aquaticus A1-9 T (98.4 %), Gemmobacterlanyuensis Orc-4 T (98.4 %), Gemmobacterfontiphilus JS43 T (98.3 %), Gemmobactercaeni DCA-1 T (98.2 %), Gemmobacternanjingensis Y12 T (97.5 %) and Gemmobactertilapiae Ruye-53 T (97.2 %). Average nucleotide identity values between the genome sequences of strain YJ-T1-11 T and the related type strains ranged from 77.08 to 90.48 %. The predominant fatty acid of strain YJ-T1-11 T was summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c). The major isoprenoid quinone was Q-10 and the DNA G+C content was 65.6 mol%. The polar lipid profile consisted of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, diphosphatidylglycerol and three unidentified lipids. The DNA-DNA relatedness values between strain YJ-T1-11 T and the type strains of the 12 phylogenetically related species of the genus Gemmobacter were 23.6-53.7 %. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain YJ-T1-11 T is considered to represent a novel species of the genus Gemmobacter, for which the name Gemmobacter lutimaris sp. nov. is proposed. The type strain is YJ-T1-11 T (=KCTC 62715 T =JCM 32828 T ).