Open AccessAlkalilacustris brevis gen. nov., sp. nov., isolated from a soda lake
Author(s) -
Ran Zhang,
Zhao Ju,
Shuai-Bo Han,
Xinjun Hou,
Yanghuan Yu,
Xinqi Zhang,
Yanghui Ye,
Cong Sun,
Guishan Zhang,
Min Wu
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003373
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , phosphatidylglycerol , strain (injury) , glycolipid , bacteria , botany , chemotaxonomy , microbiology and biotechnology , phylogenetics , taxonomy (biology) , phosphatidylcholine , biochemistry , phospholipid , gene , genetics , anatomy , membrane
A Gram-stain-negative, aerobic, non-pigmented and short-rod-shaped bacterium, designated 34079 T , was isolated from a water sample of a soda lake in Jilin, a province of China. Strain 34079 T grew at 10-50 °C (optimum, 35 °C), pH 7-10 (optimum, pH 8.0-8.5). NaCl was required for growth at the concentration range 1-10.0 % (w/v), with an optimum at 2.5-4 % (w/v). Chemotaxonomic analysis indicated that the sole respiratory quinone was Q-10. The predominant cellular fatty acids (>5 %) were summed feature 8 (C18 : 1 ω7c/C18 : 1 ω6c) and C16 : 0. The major polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, three unidentified amino lipids, one unidentified amino phosphoglycolipid, one phosphoglycolipid, one unidentified glycolipid, three unidentified phospholipids and two unidentified lipids. The DNA G+C content was 65.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 34079 T formed a distinct lineage in the clade of the family 'Rhodobacteraceae' with the highest sequence similarity of 96.1 % to Pararhodobacter aggregans, followed by Rhodobaca bogoriensis DSM 18756 T (95.7 %) and Roseibaca ekhonensis DSM 11469 T (94.7 %). The distinct biochemical, chemotaxonomic and phylogenetic differences from the previously described taxa supported that strain 34079 T represents a novel species of a new genus, for which the name Alkalilacustris brevis gen. nov., sp. nov. is proposed. The type strain is 34079 T (=KCTC 62428 T =MCCC 1K03493 T ).