Open AccessRhodophyticola porphyridii gen. nov., sp. nov., isolated from a red alga, Porphyridium marinum
Author(s) -
Hye Su Jung,
Sang Eun Jeong,
Byung Hee Chun,
ZheXue Quan,
Che Ok Jeon
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003371
Subject(s) - 16s ribosomal rna , biology , strain (injury) , phylogenetic tree , microbiology and biotechnology , bacteria , gene , genetics , anatomy
A Gram-stain-negative, strictly aerobic and moderately halophilic bacterium, designated strain MA-7-27 T , was isolated from a marine red alga, Porphyridium marinum, in the Republic of Korea. The cells of strain MA-7-27 T were non-motile rods showing oxidase- and catalase-positive activities. Growth of strain MA-7-27 T was observed at 15-45 °C (optimum, 30 °C), pH 5.0-9.0 (pH 7.0) and in the presence of 0.0-5.0 % (w/v) NaCl (2.0 %). Strain MA-7-27 T contained C10 : 0, summed feature 1 (comprising iso-C15 : 1 h and/or C13 : 1 3-OH) and summed feature 8 (comprising C18 : 1 ω7c and/or C18 : 1 ω6c) as the major fatty acids. The only isoprenoid quinone detected was ubiquinone-10. The major polar lipids of strain MA-7-27 T were phosphatidylglycerol, two unidentified phospholipids and two unidentified aminolipids. The G+C content of the genomic DNA was approximately 63.6 mol%. Strain MA-7-27 T was most closely related to the type strains of Boseongicola aestuarii BS-W15 T and Nioella nitratireducens SSW136 T with 96.98 % and 96.12 % 16S rRNA gene sequence similarities, respectively, but phylogenetic analyses showed that strain MA-7-27 T formed a clearly distinct phylogenic lineage from the closely related strains. The phenotypic, chemotaxonomic and molecular properties support that strain MA-7-27 T represents a novel genus of the family Rhodobacteraceae, for which the name Rhodophyticola porphyridii gen. nov., sp. nov. is proposed. The type strain is MA-7-27 T (=KACC 18805 T =JCM 31537 T ).