Open AccessChryseobacterium aureum sp. nov., isolated from the Han River, Republic of Korea
Author(s) -
Jueun Lee,
Eun-Mi Hwang,
Chang-Jun Cha,
GeunBae Kim
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003370
Subject(s) - biology , 16s ribosomal rna , microbiology and biotechnology , phylogenetic tree , chryseobacterium , taxonomy (biology) , strain (injury) , bacteria , botany , gene , genetics , anatomy
A Gram-stain-negative, yellow-pigmented, non-motile, non-spore-forming, aerobic and rod-shaped bacterial strain, designated 17S1E7 T , was isolated from the Han River, Republic of Korea, and characterized by polyphasic taxonomy analyses. Strain 17S1E7 T grew optimally on tryptic soy agar at 37 °C and pH 7.0 in the absence of NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain 17S1E7 T belonged to the genus Chryseobacterium and was most closely related to Chryseobacterium culicis DSM 23031 T (98.54 %). The average nucleotide identity value of strain 17S1E7 T was 91.1 % to Chryseobacterium culicis DSM 23031 T , which was lower than the cut-off of 95-96 %. The DNA G+C content of strain 17S1E7 T was 37.4 mol%. Flexirubin-type pigments were produced. The predominant respiratory quinone was menaquinone 6. The major fatty acids of strain 17S1E7 T were iso-C15 : 0, summed feature 9 (iso-C17 : 1ω9c and/or C16 : 0 10-methyl), iso-C17 : 0 3-OH and summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c). The predominant polar lipid was phosphatidylethanolamine. Based on polyphasic taxonomy data, strain 17S1E7 T represents a novel species of the genus Chryseobacterium, for which the name Chryseobacterium aureum sp. nov. is proposed. The type strain is 17S1E7 T (=KACC 19920 T =JCM 33165 T ).