Halioglobus sediminis sp. nov., isolated from coastal sediment

A novel aerobic, Gram-stain-negative, non-motile and rod-shaped bacterium, designated strain U0301 T , was isolated from a marine sediment sample in Weihai, China. This strain grew optimally at 33 °C, pH 7.5 and in the presence of 2.0-3.0 % (w/v) NaCl. Strain U0301 T shared pairwise 16S rRNA gene sequence similarities of 95.5, 95.4, 95.2 and 95.0 % to Parahaliea aestuarii S2-26 T , Halioglobus pacificus KCTC 23430 T , Halioglobus lutimaris HF004 T and Halioglobus japonicus KCTC 23429 T , respectively. Phylogenetic analysis based on the 16S rRNA gene sequences demonstrated that U0301 T formed a tight phylogenetic lineage with type strains of H. pacificus and H. japonicus. The percentage of conserved protein values of strain U0301 T as compared with H. japonicus KCTC 23429 T and Parahalieamediterranea 7SM29 T were 62.5 and 58.0 %, respectively. The ANI values of strain U0301 T with H. japonicus KCTC 23429 T and P. mediterranea 7SM29 T were 78.2 and 75.3 %, respectively. Both metrics of genome comparison suggested that strain U0301 T showed higher homology with the genus Halioglobus than the genus Parahaliea. The strain contained ubiquinone 8 as the sole respiratory quinone. The major fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and C17 : 1ω8c. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content was 61.7 mol%. Considering the phenotypic characteristics, chemotaxonomic data and phylogenetic analysis comprehensively, strain U0301 T should represent a novel species of the genus Halioglobus, for which the name of Halioglobus sediminis sp. nov. is proposed. The type strain is U0301 T (=KCTC 62082 T =MCCC 1H00234 T ).