Open AccessLactobacillus zhachilii sp. nov., a lactic acid bacterium isolated from Zha-Chili
Author(s) -
Zhendong Zhang,
Qiangchuan Hou,
Yurong Wang,
Weicheng Li,
Huijun Zhao,
Zhihong Sun,
Zhuang Guo
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003362
Subject(s) - biology , microbiology and biotechnology , bacteria , lactobacillus , lactic acid , genetics
Strain HBUAS52074 T is a Gram-positive staining, aerobic bacterium that was isolated from Zha-Chili, a traditional fermented food made in China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HBUAS52074 T is a member of the genus Lactobacillus and closely related to Lactobacillus nantensis DSM 16982 T (98.9 %), Lactobacillus heilongjiangensis DSM 28069 T (98.8 %), Lactobacillus formosensis NBRC 1095009 T (98.6 %), Lactobacillus futsaii JCM 17355 T (98.5 %), Lactobacillus farciminis KCTC 3681 T (98.5 %), Lactobacillus musae NBRC 112868 T (98.5 %) and Lactobacillus crustorum LMG 23699 T (98.4 %). The DNA G+C content is 36.3 mol%. The major cellular fatty acids are C16 : 0 (28.2 %), C18 : 1ω9c (30.5 %) and summed feature 7 (C19 : 1ω6c, and/or C19 : 1ω7c; 14.9 %). Average nucleotide identity and DNA-DNA hybridization (GGDC) values based on genomic comparisons between HBUAS52074 T and related type species showed that the bacterium was significantly different from its closest relatives. Using polyphasic taxonomic analysis, we have shown that strain HBUAS52074 T is a new species in the genus Lactobacillus, for which we propose the name Lactobacilluszhachilii sp. nov. The type strain is HBUAS52074 T (=GDMCC 1.1417 T =KCTC 21106 T ).