Open AccessPigmentiphaga humi sp. nov., isolated from soil amended with humic acid
Author(s) -
Peter Kämpfer,
HansJürgen Busse,
Alexis Criscuolo,
Chantal Bizet,
Dominique Clermont,
John A. McInroy,
Stefanie P. Glaeser
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003355
Subject(s) - biology , 16s ribosomal rna , diamino acid , phylogenetic tree , peptidoglycan , microbiology and biotechnology , strain (injury) , ribosomal rna , phylogenetics , sequence analysis , phosphatidylglycerol , type species , fatty acid , taxonomy (biology) , bacteria , botany , gene , gene sequence , genetics , biochemistry , anatomy , phospholipid , membrane , phosphatidylcholine
A slightly beige-pigmented, Gram-stain-negative, rod-shaped bacterium, strain IMT-318 T , was isolated from soil in a field located in Malvern, Alabama, USA. Phylogenetic analysis based on the 16S rRNA gene placed the strain within the genus Pigmentiphaga with highest 16S rRNA gene sequence similarity of 98.74 % and 98.67 % to the type strains of Pigmentiphaga kullae and Pigmentiphaga daeguensis, respectively. Sequence similarities to all other species of the genus were below 98.0 %. Results of the chemotaxonomic analysis, however, showed clear similarities to the genus Pigmentiphaga. The main cellular fatty acids of the strain were C16 : 0, C18 : 1 ω7c, C17 : 0 cyclo and C19 : 0 cyclo ω8c. The major quinone was ubiquinone Q-8. The polar lipid profile was composed of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified aminophospholipid. In the polyamine pattern, putrescine and 2-hydroxyputrescine were predominant. The diamino acid of the peptidoglycan was meso-diaminopimelic acid. Based on phylogenetic, chemotaxonomic and phenotypic analyses, we propose a new species of the genus Pigmentiphaga, with the name Pigmentiphaga humi sp. nov. and strain IMT-318 T (=LMG 30658 T =CIP 111626 T =CCM 8859 T ) as the type strain.