Open AccessDescription of novel members of the family Sphingomonadaceae: Aquisediminimonas profunda gen. nov., sp. nov., and Aquisediminimonas sediminicola sp. nov., isolated from freshwater sediment
Author(s) -
Long Jin,
So-Ra Ko,
Chun-Zhi Jin,
FengJie Jin,
Taihua Li,
ChiYong Ahn,
HeeMock Oh,
Hyung-Gwan Lee
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003347
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , genus , strain (injury) , lineage (genetic) , ribosomal rna , gene sequence , botany , gene , genetics , anatomy
Two Gram-stain-negative bacterial strains, DS48-3 T and CH68-4 T , were isolated from freshwater sediment taken from the Daechung Reservoir, Republic of Korea. Cells of strains DS48-3 T and CH68-4 T were aerobic, non-motile, non-spore-forming and rod-shaped. Strain DS48-3 T was isolated from a sediment surface sample at a depth of 48 m from the Daechung Reservoir and was most closely related to the genus Sphingopyxis according to 16S rRNA gene sequence analysis (94.5-95.9 % similarity). Strain CH68-4 T was isolated from the very bottom of a 67-cm-long sediment core collected from Daechung Reservoir at a water depth of 17 m and was most closely related to the genus Sphingopyxis (16S rRNA gene sequence similarity of 93.7-95.0 %). Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the two strains formed a separate lineage within the order Sphingomonadales showing similarity values below 95.9 % with their closest phylogenetic neighbours, and sharing 97.3 % similarity with each other. The combined genotypic and phenotypic data showed that strains DS48-3 T and CH68-4 T could be distinguished from all genera within the family Sphingomonadaceae and represented two distinct species of a novel genus, Aquisediminimonas profunda gen. nov., sp. nov. (type strain DS48-3 T =KCTC 52068 T =CCTCC AB 2018061 T ) and Aquisediminimonas sediminicola sp. nov. (type strain CH68-4 T =KCTC 62205 T =CCTCC AB 2018062 T ).