Open AccessLactobacillus suantsaii sp. nov., isolated from suan-tsai, a traditional Taiwanese fermented mustard green
Author(s) -
JongShian Liou,
ChienHsun Huang,
Chunlin Wang,
Ai-Yun Lee,
Koji Mori,
Tomohiko Tamura,
Mizuki Watanabe,
Jochen Blom,
Lina Huang,
Koichi Watanabe
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003346
Subject(s) - biology , 16s ribosomal rna , lactobacillus , microbiology and biotechnology , strain (injury) , sequence analysis , bacteria , gene , genetics , anatomy
A Gram-stain-positive, rod-shaped, non-motile, catalase-negative and facultative anaerobic strain, L88 T , was isolated from suan-tsai, a traditional Taiwanese fermented mustard green. Comparative analyses of 16S rRNA, pheS and rpoA gene sequences demonstrated that strain L88 T was a member of the genus Lactobacillus. On the basis of 16S rRNA gene sequence similarity, the type strains of Lactobacillus acidifarinae (98.2 % similarity), Lactobacillus namurensis (98.1 %), Lactobacillus zymae (98.1 %) and Lactobacillus spicheri (96.8 %) were the closest neighbours to this novel strain. The average nucleotide identity, digital DNA‒DNA hybridization and average amino acid identity values between L88 T and its closest relatives were lower than 80, 30 and 90 %, respectively. Phenotypic and genotypic test results demonstrated that strain L88 T represents a novel species of the genus Lactobacillus, for which the name Lactobacillus suantsaii sp. nov., is proposed. The type strain is L88 T (=BCRC 12945 T =NBRC 113535 T ).