Open AccessRoseovarius faecimaris sp. nov., isolated from a tidal flat
Author(s) -
InTae Cha,
Eui-Sang Cho,
Hak-Jong Choi,
Seong Woon Roh,
Myung-Ji Seo
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003345
Subject(s) - 16s ribosomal rna , biology , strain (injury) , phylogenetic tree , roseobacter , bacteria , phosphatidylglycerol , microbiology and biotechnology , housekeeping gene , ribosomal rna , bacterial strain , clade , botany , gene , genetics , phosphatidylcholine , phospholipid , gene expression , anatomy , membrane
A Gram-stain-negative, obligate aerobic, non-motile and rod-shaped bacterium, designated MME-070 T , was isolated from a tidal flat near Muui-do, Incheon, Republic of Korea. This bacterium belonged to the Roseobacterclade within the family Rhodobacteraceae. The isolate grew at 20-40 °C, pH 7.0-9.0 with 1-5 % (w/v) NaCl. Optimal growth required 2-3 % (w/v) NaCl at 25-30 °C and pH 8.0. The 16S rRNA gene sequence of strain MME-070 T was most closely related to Roseovarius aestuarii SMK-122 T (96.9 %). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain MME-070 T clustered with the genus Roseovarius. The sole respiratory quinone was Q-10. Polar lipids were phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, unidentified aminolipid and some unidentified lipids. Major cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), 11-methyl C18 : 1ω7c and C16 : 0. The genomic DNA G+C content of the type strain was 63.6 mol%. Based on polyphasic taxonomic analysis, strain MME-070 T is proposed to represent a novel species of the genus Roseovarius, for which the name Roseovarius faecimaris sp. nov. is proposed. The type strain is MME-070 T (=KCCM 43142 T =JCM 30757 T ).