Open AccessAzoarcus pumilus sp. nov., isolated from seawater in Sanya, China
Author(s) -
GeYi Fu,
Xiaoyun Yu,
Xiaodong Yu,
Zhe Zhao,
Can Chen,
Ruijun Wang,
Min Wu,
Xin-Qi Zhang
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003341
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , phosphatidylglycerol , genomic dna , strain (injury) , bacillus pumilus , chemotaxonomy , phylogenetics , genus , botany , microbiology and biotechnology , bacteria , gene , biochemistry , genetics , taxonomy (biology) , phospholipid , anatomy , membrane , phosphatidylcholine
A novel Gram-stain-negative, motile, rod-shaped (0.4-0.5×1.0-2.0 µm) strain with one polar flagellum, designated SY39 T , was isolated from seawater in Sanya, China. Strain SY39 T was able to grow at 15-40 °C (optimum, 35-37 °C), pH 6.5-8.5 (pH 8.0) and 0.5-6.0 % (w/v) NaCl (3.5 %). Chemotaxonomic analysis showed that the isoprenoid quinones were Q-8 (88.6 %) and Q-7 (11.4 %). The dominant fatty acids were C16 : 0 and summed feature 3 (C16 : 1ω7c/C16 : 1ω6c). The polar lipids of strain SY39 T consisted of diphosphatidyglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unknown phosphoglycolipid, one unknown glycolipid and two unknown aminophosphoglycolipids. The DNA G+C content of the genomic DNA was 66.5 mol%. The phylogenetic analysis of 16S rRNA gene sequences showed that strain SY39 T belongs to the genus Azoarcus with similarity ranging from 92.3 to 95.2 %. Based on the phenotypic, chemotaxonomic and phylogenetic features, strain SY39 T is concluded to represent a novel species of the genus Azoarcus, for which the name Azoarcus pumilus sp. nov. is proposed. The type strain is SY39 T (=KCTC 62157 T =MCCC 1K03430 T ).