Open AccessMarmoricola mangrovicus sp. nov., an endophytic actinobacterium isolated from Kandelia candel
Author(s) -
Fei-Na Li,
Zhong-Ke Jiang,
Shaowei Liu,
Tuo Li,
Simon MingYuen Lee,
ChengHang Sun
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003326
Subject(s) - biology , kandelia candel , 16s ribosomal rna , diamino acid , phylogenetic tree , strain (injury) , microbiology and biotechnology , botany , genomic dna , diaminopimelic acid , rpob , bacteria , gene , genetics , gene sequence , mangrove , ecology , anatomy
A Gram-stain-positive, aerobic, motile, non-spore-forming and rod-shaped actinobacterium, designated strain 4Q3S-7 T , was isolated from a piece of surface-sterilized bark of Kandelia candel collected at the Cotai Ecological Zone in Macao, China. Colonies were yellowish white, circular, smooth and convex. The 16S rRNA gene sequence of strain 4Q3S-7 T exhibited highest similarities to Marmoricola ginsengisoli Gsoil 097 T (97.6 %), Marmoricola solisilvae KIS18-7 T (97.6 %) and Marmoricola pocheonensis Gsoil 818 T (97.3 %). Phylogenetic analysis showed that strain 4Q3S-7 T clustered with species of the genus Marmoricola and was clearly affiliated to the genus Marmoricola. Genomic analyses, including average nucleotide identity and DNA-DNA hybridization, clearly separated strain 4Q3S-7 T from M. ginsengisoli Gsoil 097 T , M. solisilvae KIS18-7 T and M. pocheonensis Gsoil 818 T with values below the thresholds for species delineation. Strain 4Q3S-7 T had ll-2,6-diaminopimelic acid as the diagnostic diamino acid in the cell wall. The major fatty acids (>10 % of total fatty acids) were C18 : 0 10-methyl (TBSA), C18 : 1ω9c, iso-C16 : 0 and iso-C16 : 0 2-OH. The predominant menaquinone was MK-8(H4). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and an unidentified phospholipid. The DNA G+C content of strain 4Q3S-7 T was 72.0 mol% (draft genome sequence). Based on its phylogenetic, phenotypic and chemotaxonomic features, strain 4Q3S-7 T is considered to represent a novel species of the genus Marmoricola, for which the name Marmoricola mangrovicus sp. nov. is proposed. The type strain is 4Q3S-7 T (=KCTC 39790 T =CGMCC 4.7424 T ).