Open AccessBosea psychrotolerans sp. nov., a psychrotrophic alphaproteobacterium isolated from Lake Michigan water
Author(s) -
Richard A. Albert,
Molly McGuine,
Shawn C. Pavlons,
Jon Roecker,
Jennifer Bruess,
Shane Mossman,
Sona Sun,
Mike King,
Sun Hee Hong,
Christine E. Farrance,
Joseph Danner,
Yochan Joung,
Nicole Shapiro,
William B. Whitman,
HansJürgen Busse
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003319
Subject(s) - biology , 16s ribosomal rna , strain (injury) , microbiology and biotechnology , bacteria , phosphatidylglycerol , genomic dna , gene , phosphatidylcholine , genetics , phospholipid , membrane , anatomy
Three strains of a Gram-stain negative bacterium were isolated from Lake Michigan water. 16S rRNA gene sequence analysis revealed that strain 1131 had sequence similarities to Bosea vaviloviae LMG 28367 T , Bosea lathyri LMG 26379 T , Bosea lupini LMG 26383 T , Bosea eneae CCUG 43111 T , Bosea vestrisii CCUG 43114 T and Boseamassiliensis CCUG 43117 T of 99.8, 99.1, 98.4, 98.4, 98.4 and 98.2 %, respectively. The average nucleotide identity value between strain 1131 T and Bosea vaviloviae Vaf-18 T was 93.4 % and the DNA relatedness was 38 %. The primary cellular fatty acids of strain 1131 T were C16 : 1ω7c and C18 : 1ω7c. The primary polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. The major compound in the quinone system was ubiquinone Q-10 and in the polyamine pattern sym-homospermidine was predominant. Additional phenotypic characteristics included growth at 5-35 °C, pH values of pH 5.5-8.0, a salt tolerance range of 0.0-1.2 % (w/v), and production of an unknown water soluble brown pigment. After phenotypic, chemotaxonomic and genomic analyses, this isolate was identified as a novel species for which the name Bosea psychrotolerans is proposed. The type strain is 1131 T (NRRL B-65405=LMG 30034).