Open AccessTamlana carrageenivorans sp. nov., a carrageenan-degrading bacterium isolated from seawater
Author(s) -
Jaejoon Jung,
Seung Seob Bae,
Dawoon Chung,
Kwang Hyun Baek
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003318
Subject(s) - biology , seawater , bacteria , microbiology and biotechnology , carrageenan , marine bacteriophage , ecology , food science , genetics
A Gram-stain-negative, aerobic, non-motile, rod-shaped, agarolytic and carrageenolytic bacterial strain, designated UJ94 T , was isolated from seawater of Uljin in the Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain UJ94 T shared sequence similarities of 98.4, 96.1 and 95.4 % with Tamlana agarivorans JW-26 T , Tamlana sedimentorum KMM 9545 T and Tamlana crocina HST1-43 T , respectively. Growth of strain UJ94 T was observed at 4-37 °C and pH 6.5-8.0 in the presence of 2-9 % (w/v) NaCl. The major fatty acids of strain UJ94 T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and iso-C17 : 0 3-OH; MK-6 was the predominant menaquinone. Phosphatidylethanolamine, two unidentified aminolipids and five unidentified lipids were detected as major polar lipids. The whole circular genome comprised 4 116 543 bp and had a G+C content of 35.2 mol%. The ranges of average nucleotide identity and in silico DNA-DNA hybridization estimated by genome-to-genome distance were 90.6-74.2 % and 47.6-14.6 %, respectively, with the type strains of T. agarivorans and T. sedimentorum. The present polyphasic study, including phylogenetic, chemotaxonomic, biochemical and genomic data, suggested that strain UJ94 T represents a novel species of the genus Tamlana, for which the name Tamlana carrageenivorans sp. nov. is proposed. The type strain is UJ94 T (=KCTC 62451 T =NBRC 113234 T ).