Open AccessPseudomonas asiatica sp. nov., isolated from hospitalized patients in Japan and Myanmar
Author(s) -
Mari Tohya,
Shin Watanabe,
Kanae Teramoto,
Kohei Uechi,
Tatsuya Tada,
Kyoko KuwaharaArai,
Takeshi Kinjo,
Shiro Maeda,
Isamu Nakasone,
Ni Ni Zaw,
San Mya,
Khin Nyein Zan,
Htay Htay Tin,
Jiro Fujita,
Teruo Kirikae
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003316
Subject(s) - rpob , biology , housekeeping gene , 16s ribosomal rna , pseudomonas putida , microbiology and biotechnology , pseudomonas , phylogenetic tree , strain (injury) , pseudomonas stutzeri , bacteria , gene , genetics , anatomy , gene expression
A novel Gram-negative, aerobic, rod-shaped, non-spore-forming bacterial strain, RYU5 T , was isolated from a stool sample of an inpatient at a hospital in Okinawa, Japan. The optimal growth temperature of RYU5 T was 30 °C. Phylogenetic analysis based on the sequences of housekeeping genes, including the 16S rRNA, rpoB, rpoD and gyrB genes, showed that RYU5 T was a member of the Pseudomonas putida group and was located close to Pseudomonas monteilii and P. putida. Whole-genome comparisons, using average nucleotide identity and digital DNA-DNA hybridization, confirmed that strain RYU5 T should be classified as a novel species of Pseudomonas. Phenotypic characterization tests showed that utilization of d-mannose, d-serine, l-arabinose and d-fructose could distinguish this strain from other related species of the genus Pseudomonas. Based on genetic and phenotypic evidence, strain RYU5 T should be classified as a novel species, for which the name Pseudomonas asiatica sp. nov. is proposed. The type strain is RYU5 T (=DSM 107182 T , =JCM 32716 T ), with a DNA G+C content of 62.25 mol%.