Open AccessCroceicoccus ponticola sp. nov., a lipolytic bacterium isolated from seawater
Author(s) -
Sooyeon Park,
SungMin Won,
Tae-Kwang Oh,
JungHoon Yoon
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003301
Subject(s) - 16s ribosomal rna , biology , strain (injury) , phylogenetic tree , botany , bacteria , gene , genetics , anatomy
A lipolytic, Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated GM-16 T , was isolated from seawater around Pohang in the Republic of Korea. Strain GM-16 T grew optimally at 30 °C and in the presence of 1.0-2.0 % (w/v) NaCl. The neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain GM-16 T fell within the clade comprising the type strains of Croceicoccus species. Strain GM-16 T exhibited the highest 16S rRNA gene sequence similarity (97.7 %) to the type strain of Croceicoccus pelagius and sequence similarities of 96.3-96.7 % to the type strains of the other Croceicoccus species. Strain GM-16 T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) as major fatty acids. The major polar lipids of strain GM-16 T were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid and one unidentified glycolipid. The DNA G+C content of strain GM-16 T was 62.6 mol%. The mean DNA-DNA relatedness value between strain GM-16 T and C. pelagius DSM 101479 T was 16 %. The average nucleotide identity values between strain GM-16 T and the type strains of C.roceicoccus pelagius, C.roceicoccus marinus, C.roceicoccus naphthovorans and C.roceicoccus mobilis were 83.96-84.44 %. The phylogenetic and genetic data and differential phenotypic properties indicated that strain GM-16 T is separated from recognized Croceicoccus species. On the basis of the data presented here, strain GM-16 T is considered to represent a novel species of the genus Croceicoccus, for which the name Croceicoccus ponticola sp. nov. is proposed. The type strain is GM-16 T (=KACC 19611 T =KCTC 62423 T =NBRC 113192 T ).