Open AccessSinorhodobacter populi sp. nov., isolated from the symptomatic bark tissue of Populus × euramericana canker
Author(s) -
Guantang Xu,
Chungen Piao,
Ju-pu Chang,
Limin Guo,
Xiutao Yang,
Yong Li
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003300
Subject(s) - biology , 16s ribosomal rna , botany , canker , bark (sound) , strain (injury) , microbiology and biotechnology , bacteria , anatomy , genetics , ecology
We isolated five novel bacterial strains from symptomatic bark tissue of Populus × euramericana canker that were Gram-stain-negative, non-motile, aerobic oxidase-negative and catalase-positive. Growth occurred at 10–41 °C and at pH 5.0–7.0, with optimum growth at 30 °C and pH 7.0. Additionally, growth occurred in conditions of 0–5 % (w/v) salinity, but not above 7 % NaCl. The 16S rRNA gene sequences of the novel strains shared the highest similarity with Sinorhodobacter ferrireducens SgZ-3 T (97.1 %). The average nucleotide identity values between the novel strains and two type strains ( S.inorhodobacter ferrireducens CCTCC AB2012026 T and ‘ S inorhodobacter hungdaonensis ’ CGMCC 1.12963 T ) were 78.4–78.9 %, which were lower than the proposed species boundary cut-off (95–96 %). The main polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified lipid and phosphatidylcholine. The main respiratory quinone was Q-10, and major fatty acids were C 18 : 1 ω7 c and/or C 18 : 1 ω 6 c . Based on data from a polyphasic taxonomy study, the novel strains represent a novel species of the genus Sinorhodobacter , for which the name Sinorhodobacter populi sp. nov. is proposed. The type strain is sk2b1 T (=CFCC 14580 T =KCTC 52802 T ).