Open AccessAcuticoccus sediminis sp. nov., isolated from deep-sea sediment of the Indian Ocean and proposal of Acuticoccaceae fam. nov
Author(s) -
Qiliang Lai,
Xiupian Liu,
Fenqing Sun,
Zongze Shao
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003289
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , lineage (genetic) , strain (injury) , oxidase test , glycolipid , bacteria , clade , genomic dna , genus , gene , microbiology and biotechnology , botany , biochemistry , genetics , enzyme , anatomy
A Gram-staining negative, aerobic, oval-shaped bacterium, designated strain PTG4-2 T , was isolated from deep-sea sediment of the Indian Ocean. Growth was observed with 1–9 % (w/v) NaCl with optimal growth with 3 %, at pH 6.0–10.0 with an optimum of pH 7.0, and at 4–40 °C with an optimum of 30 °C. Positive for catalase and oxidase. The results of a 16S rRNA gene sequence comparison indicated that PTG4-2 T was most closely related to Acuticoccus yangtzensis JL1095 T (97.3 %), followed by Acuticoccus kandeliae J103 T (96.5 %), all other species shared <93 % sequence similarity. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that PTG4-2 T forms a distinct lineage within the genus Acuticoccus , and revealed that the genus Acuticoccus forms a novel family-level clade in the order Rhizobiales . The ANI and the DNA–DNA hybridization estimate values between PTG4-2 T and two type strains ( A. yangtzensis JL1095 T and A. kandeliae J103 T ) were 79.9–76.2 % and 23.1–20.8 %, respectively. PTG4-2 T contained Q-10 as the predominant ubiquinone. The principal fatty acids (>5 %) were summed feature 8 [C 18 : 1 ω 7 c / ω 6 c (72.2 %)], C 18 : 0 (8.4 %), C 20 : 1 ω 7 c (6.4 %) and C 16 : 0 (6.3 %). The polar lipids consisted of phosphatidylglycerol, three unidentified phospholipids, two unidentified glycolipids, one unidentified aminolipid and one unknown lipid. The DNA G+C content of PTG4-2 T is 69.2 mol%. On the basis of the polyphasic taxonomic evidence presented in this study, PTG4-2 T should be classified as representing a novel species of the genus Acuticoccus , for which the name Acuticoccus sediminis sp. nov. is proposed, with the type strain PTG4-2 T (=MCCC 1A01274 T =KCTC 52323 T ). In addition, a novel family, Acuticoccaceae fam. nov., is proposed to accommodate the genus Acuticoccus .