Open AccessCitroniella saccharovorans gen. nov. sp. nov., a member of the family Peptoniphilaceae isolated from a human fecal sample from a coastal traditional community member
Author(s) -
Niyati Patel,
Alexandra J. Obregón-Tito,
Raúl Y. Tito,
Omar Trujillo-Villaroel,
Luis Marin-Reyes,
Luzmila Troncoso-Corzo,
Emilio Guija-Poma,
Cecil M. Lewis,
Paul A. Lawson
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003287
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , microbiology and biotechnology , bacteria , feces , taxonomy (biology) , genus , coccus (insect) , ribosomal rna , diamino acid , gene , zoology , botany , genetics , gene sequence
A novel Gram-stain-positive, non-motile, non-spore-forming coccus-shaped obligately anaerobic bacterium was recovered from a fecal sample obtained from an individual from a traditional community located on the southern coast of Peru. The results of analysis based on 16S rRNA gene sequencing indicated the novel bacterium to be phylogenetically distinct from other genera of members of the Peptoniphilaceae family, sharing a loose affinity with the genera Ezakiella , Finegoldia , Gallicola and Parvimonas . The major cellular fatty acids of the novel isolate were determined to be C 16:0 , C 17:1 ω8 c , and C 18:1 ω9 c . The DNA G+C content was 29.9 mol%. End products of metabolism from peptone yeast glucose broth (PYG) were determined to be acetate and methyl succinate. The diagnostic diamino acid present in the cell wall was lysine. On the basis of the phenotypic, chemotaxonomic and phylogenetic results the organism is a member of a novel genus belonging to the family Peptoniphilaceae for which the name Citroniella saccharovorans gen nov. sp. nov., is proposed. The type strain is M6.X9 T (DSM 29873 T =CCUG 66799 T ).