Open AccessSinomicrobium soli sp. nov., isolated from arctic soil
Author(s) -
Xiupian Liu,
Qiliang Lai,
Yaping Du,
Xiaorong Zhang,
Zhong Huang,
Zongze Shao
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003273
Subject(s) - 16s ribosomal rna , biology , phylogenetic tree , flavobacteriaceae , strain (injury) , genus , bacteria , bacteroidetes , botany , microbiology and biotechnology , biochemistry , gene , genetics , anatomy
A bacterial strain, designated N-1-3-6 T , was isolated from a soil sample collected from the arctic regions. The cells were short rods, Gram-stain-negative, catalase- and oxidase-positive. Growth was observed with 0–12 % (w/v) NaCl, with optimal growth at 0.5–2 %, and at pH 6.0–9.0, with optimum of pH 7.0, and a growth temperature of 10–45 °C, with an optimum of 28–37 °C. Phylogenetic analysis based on the 16S rRNA gene placed N-1-3-6 T in the genus Sinomicrobium with the closest relative being Sinomicrobium pectinilyticum 5DNS001 T , exhibiting 95.3 % 16S rRNA pairwise similarity. A polyphasic taxonomic study, including phenotypic, chemotaxonomic and molecular analyses, was performed to clarify its taxonomic position. N-1-3-6 T contained MK-6 as the predominant menaquinone. Polar lipids consisted of phosphatidylethanolamine and several unidentified aminolipids, phospholipids and lipids. The principal fatty acids (>10 %) were iso-C 15 : 0 (26.9 %), summed feature 3 [C 16 : ω7c / ω6 c (17.2 %)] and iso-C 17 : 0 3-OH (14.7 %). The DNA G+C content of N-1-3-6 T was 47.7 mol%. On the basis of its phenotypic and genotypic properties, strain N-1-3-6 T should be classified as representing a novel species of the genus Sinomicrobium , for which the name Sinomicrobium soli sp. nov. is proposed, with the type strain N-1-3-6 T (=MCCC 1A06047 T =KCTC 52339 T ).