Open AccessBacillus acidinfaciens sp. nov., isolated from farmland soil
Author(s) -
Lei Sun,
Yan Chen,
Wei Tian,
Lunguang Yao,
Zhaojin Chen,
Wenkai Ouyang
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003271
Subject(s) - biology , 16s ribosomal rna , peptidoglycan , strain (injury) , phylogenetic tree , microbiology and biotechnology , diamino acid , genomic dna , bacillus (shape) , bacteria , botany , gene , biochemistry , genetics , gene sequence , anatomy
A Gram-stain-positive, rod-shaped, motile bacterial strain, designated 3-2-2 T , was isolated from field topsoil collected from a western suburb of Nanyang city, Henan province, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2 T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514 T (98.9 % similarity), Bacillus terrae RA9 T (98.0 %) and Bacillus fordii R-7190 T (97.7 %). A draft genome sequence determined for strain 3-2-2 T revealed a DNA G+C content of 42.2 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between 3-2-2 T and the closely related Bacillus species ranged 79.4–84.2 % and 23.4–24.6 %. The major fatty acids of strain 3-2-2 T were iso-C 15 : 0 , anteiso-C 15 : 0 , iso-C 14 : 0 and iso-C 16 : 0 . The major isoprenoid quinone was MK-7. meso-Diaminopimelic acid was detected in the peptidoglycan. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and an unidentified lipid. The results of phylogenetic analyses, in silico genomic comparisons, and chemotaxonomic and phenotypic analyses clearly indicated that strain 3-2-2 T represents a novel species within the genus Bacillus , for which the name Bacillus acidinfaciens sp. nov. is proposed. The type strain is 3-2-2 T (=CGMCC 1.13685 T =LMG 30839 T ).