Open AccessButyricimonas faecalis sp. nov., isolated from human faeces and emended description of the genus Butyricimonas
Author(s) -
Tiphaine Le Roy,
Patrick Van Der Smissen,
Adrien Paquot,
Nathalie M. Delzenne,
Giulio G. Muccioli,
Jean-François Collet,
Patrice D. Cani
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003249
Subject(s) - biology , 16s ribosomal rna , microbiology and biotechnology , strain (injury) , ribosomal rna , bacteria , gene , biochemistry , genetics , anatomy
A Gram-negative, strictly anaerobic, non-spore forming, non-motile, non-pigmented bacterial strain, designated H184 T , was isolated from human faeces. 16S rRNA gene sequence analysis showed that strain H184 T represents a member of the genus Butyricimonas. Strain H184 T is related to but distinct from Butyricimonasvirosa JCM 15149 T and Butyricimonasparavirosa JCM 18677 T , with 16S rRNA gene sequence similarities of 96.32 and 96.24 %, respectively. Strain H184 T shared 90.50 % hsp60 gene sequence similarity to B. virosa JCM 15149 T and B. paravirosa JCM 18677 T . Growth occurs between 25 and 42 °C with an optimum at 37 °C. Bile and NaCl concentration range allowing growth are 0-3.75 % and 0-1.8 %, respectively. pH range for growth is 5.5-8. The strain produced propionate as the major end product from glucose. The major cellular fatty acids of strain H184 T were iso-C15 : 0 (63.5 %) and iso-C17 : 0 3-OH (12.8%). The major menaquinone of the strain was MK-10 (86 %). DNA G+C content of the isolate H184 T was 44.2 mol%. The genome-based comparison between strain H184 T and B. virosa JCM 15149 T by pairwise average nucleotide identity indicated a clear distinction with a score of 87.22. On the basis of these data, strain H184 T represents a novel species of the genus Butyricimonas, for which the name Butyricimonas faecalis sp. nov. is proposed. The type strain of B. faecalis is H184 T (DSM 106867 T , LMG 30602 T ).