Open AccessMotilimonas pumila sp. nov., isolated from the gut of sea cucumber Apostichopus japonicus
Author(s) -
Feng Qing Wang,
Li Ren,
YuWei Lin,
Guo Sun,
Zong Jun Du,
Jinxin Zhao,
Xiao Jing Liu,
Li Juan Liu
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003242
Subject(s) - apostichopus japonicus , biology , sea cucumber , 16s ribosomal rna , phylogenetic tree , strain (injury) , genus , botany , phosphatidylglycerol , ribosomal rna , bacteria , microbiology and biotechnology , gene , genetics , ecology , anatomy , phospholipid , membrane , phosphatidylcholine
A facultatively anaerobic and Gram-negative bacterium, designated strain PLHSC7-2 T , was isolated from the gut of sea cucumber Apostichopusjaponicus that had been collected from the coastal area of Yantai, China. The cells were rod-shaped and motile by means of polar flagella. The novel isolate grew optimally at 28-30 °C, in the presence of 2.0-3.0 % (w/v) NaCl and at pH 7.0-7.5. The sole respiratory quinone was Q-8 and the major fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0 and C17 : 0. The predominant polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain PLHSC7-2 T was phylogenetically affiliated with the genus Motilimonas, and exhibited sequence similarity of 96.2 % toMotilimonas eburnea YH6 T . The DNA G+C content was 45.5 mol%. On the basis of phenotypic , phylogenetic and genetic distinctiveness, strain PLHSC7-2 T (=MCCC 1K03522 T =KCTC 62589 T ) was classified as a novel species within the genus Motilimonas, for which the name Motilimonas pumila sp. nov. is proposed.