Open AccessPseudaminobacter arsenicus sp. nov., an arsenic-resistant bacterium isolated from arsenic-rich aquifers
Author(s) -
Yao Mu,
Xing Kui Zhou,
Lan Liu,
Xing-Kui Zhou,
XianChun Zeng,
Wenjun Li
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003238
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , strain (injury) , arsenic , bacteria , genus , phylogenetics , microbiology and biotechnology , botany , ribosomal rna , gene , genetics , materials science , anatomy , metallurgy
An arsenic-resistant strain, CB3 T , was isolated from arsenic-rich aquifers at the Jianghan Plain in Hubei, China. Phylogenetic and biochemical analysis suggested that it should represent a new species of the genus Pseudaminobacter in the family Phyllobacteriaceae. The 16S rRNA gene of CB3 T shared the highest sequence similarities to those of the type strains Pseudaminobacter defluvii THI 051 T (97.8 % identity) and Pseudaminobacter salicylatoxidans BN12 T (97.4 %). The DNA-DNA relatedness values of CB3 T with respect to strains belonging to the genus Pseudaminobacter were less than 70 %. The fatty acid profile of CB3 T consisted of C16 : 0, cyclo-C19 : 0ω8c and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) as major components. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidyldimethylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine and diphosphatidylglycerol. The DNA G+C content was 61.4 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain CB3 T was distinct from previously described Pseudaminobacter species. Therefore, we propose that strain CB3 T represents a novel species of the genus Pseudaminobacter, Pseudaminobacterarsenicus sp. nov., strain CB3 T (=CCTCC AB2016116 T =KCTC 52625 T ) is designated as the type strain.