Open AccessCohnella faecalis sp. nov., isolated from animal faeces in a karst cave
Author(s) -
Hai-Zhen Zhu,
Xiaodong Liu,
ChengYing Jiang,
ShuangJiang Liu
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003228
Subject(s) - biology , 16s ribosomal rna , peptidoglycan , diamino acid , microbiology and biotechnology , diaminopimelic acid , strain (injury) , phylogenetic tree , genomic dna , ribosomal rna , phylogenetics , bacteria , gene , genetics , gene sequence , anatomy
A Gram-stain-positive, rod-shaped, endospore-forming, motile and aerobic bacterial isolate, designated strain K2E09-144 T , was obtained from animal faeces that were collected from a karst cave in Guizhou province, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain K2E09-144 T represents a novel member of the genus Cohnella within the family Paenibacillaceae of the phylum Firmicutes. Strain K2E09-144 T was phylogenetically closely related to Cohnella nanjingensis D45 T (16S rRNA gene sequence similarity 97.0 %). The major cellular fatty acids were anteiso-C15:0, iso-C16:0 and C16 : 0. The major isoprenoid quinone was menaqinone 7 (MK-7). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified phospholipid, four unidentified aminophospholipids, one glycolipid and one unidentified lipid. The isomer type of diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The genome of strain K2E09-144 T comprised 6.43 Mb, and encoded 6029 genes in total. The DNA G+C content of the genomic DNA was 53.3 mol%. Based on its phylogenetic, phenotypic and chemotaxonomic characteristics, strain K2E09-144 T is considered to represent a novel species of the genus Cohnella, for which the name Cohnella faecalis sp. nov. is proposed. The type strain is K2E09-144 T (=CGMCC 1.13587 T =NBRC 113454 T ).