Open AccessHistidinibacterium lentulum gen. nov., sp. nov., a marine bacterium from the culture broth of marine microalga Picochlorum sp. 122
Author(s) -
Guanghua Wang,
Shuailiang Xu,
Hongfei Su,
Biao Chen,
Jiayuan Liang,
Wen Huang,
Yinghui Wang,
Kefu Yu
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003225
Subject(s) - biology , 16s ribosomal rna , strain (injury) , microbiology and biotechnology , phylogenetic tree , bacteria , flagellum , phosphatidylglycerol , ribosomal rna , gene , phospholipid , genetics , anatomy , membrane , phosphatidylcholine
A Gram-stain-negative, non-spore-forming, aerobic, motile, ovoid or short rod shaped bacterium, designed strain B17 T , was isolated from the culture broth of Picochlorum sp. 122. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain B17 T forms a stable cluster with Oceanicolagranulosus MCCC 1A10589 T (with the highest 16S rRNA gene similarity of 95.8 %) and Roseisalinusantarcticus DSM 11466 T in the family Rhodobacteraceae. The only detected respiratory quinone was Q-10. The major cellular fatty acids were C18 : 1 ω7c/ω6c, C17 : 1iso I/anteiso B and C16 : 0. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, an unidentified phospholipid and two unidentified lipids. The genome G+C content was 69 mol%. Characteristics, such as a long lag phase, being motile with single polar flagellum, no aminolipid content, and little or no C18 : 1ω7c11-methyl and C19 : 0cyclo ω8c content could significantly distinguish strain B17 T from its closely related type strains. Therefore strain B17 T is suggested to represent a new species in a new genus, for which Histidinibacterium lentulum gen. nov., sp. nov. is proposed. The type strain B17 T (=MCCC 1K03225 T =KCTC 52553 T ).