Open AccessGlaciecola amylolytica sp. nov., an amylase-producing bacterium isolated from seawater
Author(s) -
Yao Xiao,
ZhengFei Yan,
Yoo-Sung Kim,
Hyun-Myung Lee,
Huan Trinh,
Jung Eun Yang,
KyungHwa Won,
TaeHoo Yi,
MooChang Kook
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003222
Subject(s) - 16s ribosomal rna , biology , strain (injury) , coccus (insect) , phylogenetic tree , phosphatidylglycerol , bacterial strain , bacteria , microbiology and biotechnology , gene , botany , genetics , phospholipid , anatomy , membrane , phosphatidylcholine
A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium (THG-3.7 T ) was isolated from seawater. Growth occurred at 10–30 °C (optimum 25 °C), at pH 6–8 (optimum 7) and in the presence of 1–8 % (w/v) NaCl (optimum 4 %). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-3.7 T were identified as Paraglaciecola mesophila DSM 15026 T (95.3 % similarity), Glaciecola pallidula DSM 14239 T (95.2 %), Paraglaciecola aquimarina KCTC 32108 T (95.1 %), Paraglaciecola arctica KACC 14537 T (94.9 %), Glaciecola nitratireducens KCTC 12276 T (94.7 %) and Paraglaciecola psychrophila CGMCC 1.6130 T (94.7 %). 16S rRNA gene sequence similarities among strain THG-3.7 T and other species were lower than 94.7 %. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified lipid and one unidentified aminolipid. The quinone system was composed of Q-8. The major fatty acids were C 16 : 0 , C 18 : 1 ω 7 c and summed feature 3 (C 16 : 1 ω 7 c and/or iso-C 15 : 0 2-OH). The DNA G+C content of strain THG-3.7 T was 47.9 mol%. On the basis of the data presented, strain THG-3.7 T represents a novel species of the genus Glaciecola , for which the name Glaciecola amylolytica sp. nov. is proposed. The type strain is THG-3.7 T (=KACC 19478 T =CCTCC AB 2017258 T ).