Open AccessDyella solisilvae sp. nov., isolated from mixed pine and broad-leaved forest soil
Author(s) -
Zeng-hong Gao,
Zi Jiang Yang,
Meihong Chen,
Zhijie Huang,
Li-hong Qiu
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003218
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phylogenetic tree , botany , genus , flagellum , clade , housekeeping gene , phylogenetics , gene , genetics , anatomy , gene expression
A Gram-stain-negative, aerobic, motile, yellow-pigmented, rod-shaped with a single polar flagellum bacterial strain, designated strain DHG54 T , was isolated from a forest soil sample of Dinghushan Biosphere Reserve, Guangdong Province, China. Strain DHG54 T grew at 12–37 °C (optimum, 28 °C), pH 4.5–8.0 (optimum, pH 6.0–7.0) and in the presence of 0–3.0 % (w/v) NaCl (optimum, 0–1.5 %, w/v). Based on 16S rRNA gene sequence analysis, strain DHG54 T formed a clade with the members of the genus Dyella and showed highest sequence similarities of 98.2 % to Dyella japonica DSM 16301 T and Dyella terrae KACC 12748 T . This was also supported by phylogenetic analysis based on the concatenated partial gyrB , lepA and recA housekeeping gene sequences. DNA–DNA hybridization results between strain DHG54 T and closely related Dyella species were all lower than 70 %. Ubiquinone-8 was the only respiratory quinone, and iso-C 15 : 0 , iso-C 17 : 0 and iso-C 17 : 1 ω 9 c were major fatty acids. The DNA G+C content of strain DHG54 T was 65.4 mol%. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. On the basis of the polyphasic characterization results presented here, strain DHG54 T represents a novel species of the genus Dyella , for which the name Dyella solisilvae sp. nov. (type strain DHG54 T =GDMCC 1.1187 T = LMG 30091 T ) is proposed.