Open AccessZavarzinia aquatilis sp. nov., isolated from a freshwater river
Author(s) -
Yunho Lee,
Hye Yoon Park,
Che Ok Jeon
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003214
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , strain (injury) , microbiology and biotechnology , bacteria , oxidase test , gene , sequence analysis , genetics , biochemistry , enzyme , anatomy
A Gram-stain-negative, strictly aerobic, catalase-positive and oxidase-positive bacterium, designated strain HR-AS T , was isolated from a water sample of the Han River located in the Republic of Korea. Cells were motile rods with a polar flagellum. Growth was observed at 5-35 °C (optimum of 25 °C) and pH 6-8 (optimum of pH 7) and in the presence of 0-2 % (w/v) NaCl (optimum of 0 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HR-AS T formed a tight phylogenic lineage with Zavarzinia compransoris LMG 5821 T . Strain HR-AS T was most closely related to Z. compransoris LMG 5821 T with a 98.7 % 16S rRNA gene sequence similarity and had very low similarities (below 91.0 %) to other type strains with validly published names. Average nucleotide identity and in silico DNA-DNA hybridization values between strain HR-AS T and Z. compransoris DSM 1231 T were 80.4 and 23.1 %, respectively. Strain HR-AS T contained ubiquinone-10 as the major quinone and homospermidine and putrescine as the major polyamines. The major fatty acids were summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c), C16 : 0 and C18 : 1 2-OH. Strain HR-AS T contained diphosphatidylglycerol, an unidentified aminolipid and two unidentified phospholipids as major polar lipids. The DNA G+C content of strain HR-AS T was 67.2 mol%. Based on the genotypic, chemotaxonomic and phenotypic analyses, strain HR-AS T represents a novel species of the genus Zavarzinia, for which the name Zavarziniaaquatilis sp. nov. is proposed. The type strain is HR-AS T (=KACC 19412 T =JCM 32263 T ).