Open AccessStreptomyces inhibens sp. nov., a novel actinomycete isolated from rhizosphere soil of wheat (Triticum aestivum L.)
Author(s) -
Liying Jin,
Yang Zhao,
Wei Song,
Liping Duan,
Shuting Jiang,
Xiangjing Wang,
Junwei Zhao,
Wensheng Xiang
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003204
Subject(s) - biology , 16s ribosomal rna , rhizosphere , botany , streptomyces , rpob , strain (injury) , microbiology and biotechnology , gene , genetics , bacteria , anatomy
A novel actinomycete, designated strain NEAU-D10 T , was isolated from rhizosphere soil of wheat (Triticum aestivum L.) collected from Northeast Agricultural University in Harbin, Heilongjiang Province, north-east China. A polyphasic approach was employed to determine the status of strain NEAU-D10 T . Morphological and chemotaxonomic characteristics were consistent with those of members of the genus Streptomyces. The menaquinones detected were MK-9 (H6), MK-9 (H8) and MK-9 (H4). The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol and three unidentified lipids. The major fatty acids were identified as iso-C16 : 0, C16 : 0, anteiso-C15 : 0 and iso-C14 : 0. Analysis of the 16S rRNA gene sequence showed that strain NEAU-D10 T belongs to the genus Streptomyces with high sequence similarity to Streptomyces sioyaensis DSM 40032 T (99.0 %) and Streptomyces auratus DSM 41897 T (98.8 %). Moreover, multilocus sequence analysis based on five other housekeeping genes (atpD, gyrB, rpoB, recAand trpB) and the low level of DNA-DNA relatedness and phenotypic differences allowed the novel isolate to be differentiated from its most closely related strains, S. sioyaensis DSM 40032 T and S. auratus DSM 41897 T . It is concluded that the organism can be classified as representing a novel species of the genus Streptomyces, for which the name Streptomycesinhibens sp. nov. is proposed. The type strain is NEAU-D10 T (=CGMCC 4.7469 T =DSM 106197 T ).