Open AccessMicrobacterium ureisolvens sp. nov., isolated from a Yellow River sample
Author(s) -
Li-Jiao Cheng,
Ming Hong,
Zhuo-Li Zhao,
Wei-Li Ji,
Lingyu Zhang,
Lanyu Li,
Xiaolin Meng,
Meng Li,
Mingming Niu,
Guoxing Nie
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003203
Subject(s) - biology , 16s ribosomal rna , microbacterium , microbiology and biotechnology , strain (injury) , phylogenetic tree , peptidoglycan , actinomycetales , phylogenetics , bacteria , gene , genetics , streptomyces , anatomy
A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain, CFH S00084 T , was isolated from a sediment sample of the Yellow River in Henan Province, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084 T clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959 T and Microbacterium arthrosphaerae DSM 22421 T (98.97 % and 98.36 % similarity, respectively). The strain grew optimally at 25-37 °C, at pH 7.0 and in 0-3 % (w/v) NaCl. The major whole-cell sugars were rhamnose and glucose. The cell-wall peptidoglycan mainly contained glycine, alanine and ornithine. The menaquinones of strain CFH S00084 T were MK-13, MK-12 and MK-11. The major fatty acids detected were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The genome of strain CFH S00084 T was 4.03 Mbp with a G+C content of 70.5 mol%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH S00084 T and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %). The phylogenomic analysis provided evidence for clear phylogenetic divergence between strain CFH S00084 T and its closely related type strains. On the basis of the differential physiological properties, chemotaxonomic characteristics and low ANI and dDDH results, strain CFH S00084 T is considered to represent a novel species for which the name Microbacteriumureisolvens sp. nov. is proposed. The type strain is CFH S00084 T (=KCTC 39802 T =DSM 103157 T ).