Open AccessAmycolatopsis panacis sp. nov., isolated from Panax notoginseng rhizospheric soil
Author(s) -
Guang Peng,
Deng-Sen Xiong,
Lin-Chao Li,
Jiayun Hu,
Sheng Bao,
YouWei Chen,
Yiqing Li,
Li-Hua Xu,
CuiPing Miao,
LiXing Zhao
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003202
Subject(s) - biology , 16s ribosomal rna , microbiology and biotechnology , strain (injury) , genomic dna , bacteria , diamino acid , peptidoglycan , phylogenetic tree , botany , gene , biochemistry , gene sequence , genetics , anatomy
A novel Gram-positive bacterium, designated strain YIM PH21725 T , was isolated from a sample of rhizospheric soil of Panaxnotoginseng cultivated in Anning, Yunnan. The strain contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The main fatty acids identified were C17 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 0. The main menaquinone was MK-9 (H4). The polar lipids included phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, phospholipids and phospholipids of an unidentified structure containing glucosamine. The G+C content of genomic DNA was 69.43 mol%. On the basis of its 16S rRNA gene sequence, strain YIM PH21725 T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092 T (98.57 %), Amycolatopsis jejuensis JCM13280 T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885 T (96.88 %) and Amycolatopsis ultiminotia JCM 16989 T (96.8 %). The phenotypic, chemotaxonomic, phylogenetic and digital DNA-DNA hybridization results clearly indicated that strain YIM PH21725 T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsispanacis sp. nov. is proposed. The type strain is YIM PH21725 T (=CCTCC AA 2017044 T =KCTC 49031 T =DSM 105902 T ).