Open AccessZhongshania marina sp. nov., isolated from deep-sea water
Author(s) -
Young Gwon On,
Jaeseong Oh,
Dong-Hyun Roh
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003195
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phosphatidylglycerol , flagellum , genomic dna , botany , oxidase test , microbiology and biotechnology , bacteria , gene , biochemistry , genetics , anatomy , phospholipid , membrane , phosphatidylcholine , enzyme
A Gram-stain-negative, aerobic, catalase-positive, oxidase-positive, motile by a single polar flagellum, rod-shaped strain, designated DSW25-10 T , was isolated from the deep-sea water of the East Sea, Republic of Korea. Strain DSW25-10 T grew at 4-35 °C (optimum, 20-35 °C), at pH 5.5-9.0 (optimum, pH 7.0) and in the presence of 0-6.0 % NaCl (optimum, 0.5-2 %), and could assimilate valerate, but not assimilate d-mannitol and 3-hydroxy-butyrate. Comparative 16S rRNA gene sequence analysis showed that strain DSW25-10 T belongs to the genus Zhongshania in the family Spongiibacteraceae and is most closely related to Zhongshania guokunii ZS6-22 T , Zhongshania borealis CL-AS9 T , Zhongshania aliphaticivorans SM-2 T and Zhongshani antarctica ZS5-23 T with a similarities of 97.1, 97.0, 96.7 and 96.6 %, respectively. The G+C content of genomic DNA was 49.1 mol% and the major respiratory quinone was ubiquinone-8. Phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol were identified as the major cellular polar lipids. The predominant cellular fatty acids in strain DSW25-10 T were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c), C16 : 0 and C17 : 1ω8c. The DNA-DNA relatedness values between strain DSW25-10 T and related strains were clearly lower than 70 %. On the basis of evidence from a polyphasic analysis, strain DSW25-10 T is proposed to represent a novel species, Zhongshania marina sp. nov. The type strain is DSW25-10 T (=KCCM 43273 T =JCM 17372 T ).