Open AccessTransfer of the waterfall source isolate Pectobacterium carotovorum M022 to Pectobacterium fontis sp. nov., a deep-branching species within the genus Pectobacterium
Author(s) -
Saïd Oulghazi,
Jérémy Cigna,
Yin Yin Lau,
Mohiéddine Moumni,
KokGan Chan,
Denis Faure
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003180
Subject(s) - biology , pectobacterium , pectobacterium carotovorum , housekeeping gene , genetics , multilocus sequence typing , genomic dna , microbiology and biotechnology , botany , gene , genotype , gene expression
Pectobacterium carotovorum M022 T has been isolated from a waterfall source in Selangor district (Malaysia). Using genomic and phenotypic tests, we re-examined the taxonomical position of this strain. Based on 14 concatenated housekeeping genes (fusA, rpoD, rpoS, acnA, purA, gyrB, recA, mdh, mtlD, groEL, secY, glyA, gapA and rplB), multi-locus sequence analysis revealed that strain M022 T falls into a novel clade separated from the other Pectobacterium species. The in silico DNA-DNA hybridization and average nucleotide identity values were lower than the 70 and 95 % threshold values, respectively. In addition, by combining genomic and phenotypic tests, strain M022 T may be distinguished from the other Pectobacterium isolates by its incapacity to grow on d(+)-xylose, l-rhamnose, cellobiose and lactose. Strain M022 T (=CFBP 8629 T =LMG 30744 T ) is proposed as the type strain of the Pectobacteriumfontis sp. nov.