Open AccessCrenobacter cavernae sp. nov., isolated from a karst cave, and emended description of the genus Crenobacter
Author(s) -
Hai-Zhen Zhu,
ChengYing Jiang,
ShuangJiang Liu
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003179
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , genus , genomic dna , phylogenetics , strain (injury) , cave , botany , proteobacteria , gene , taxonomy (biology) , microbiology and biotechnology , genetics , ecology , anatomy
A Gram-stain-negative, rod-shaped, motile and strictly aerobic novel bacterial isolate, designated strain K1W11S-77 T , was obtained from a water sample that was collected from a karst cave in Guizhou province, PR China. The results of a phylogenetic analysis based on 16S rRNA gene sequences indicated that K1W11S-77 T represented a member of the genus Crenobacter within the family Neisseriaceae of the phylum Proteobacteria. K1W11S-77 T was phylogenetically closely related to Crenobacter luteusYIM 78141 T (Their 16S rRNA gene sequence similarity is 95.02 %). Growth of K1W11S-77 T occurred at 10-30 °C, at pH 7-9, and in the presence of 0-1 % (w/v) NaCl. The major cellular fatty acids were C12 : 0, C16 : 0, C18:1ω7c and summed feature 3. The major isoprenoid quinone was Q-8. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and one unidentified phospholipid. The genome of K1W11S-77 T was 3.27 Mb long and encoded 3167 annotated genes. The DNA G+C content of the genomic DNA was 65.3 mol%. On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, K1W11S-77 T is considered to represent a novel species of the genus Crenobacter, for which the name Crenobactercavernae sp. nov. is proposed. The type strain is K1W11S-77 T (=CGMCC 1.13527 T =NBRC 113452 T ).