Open AccessRufibacter sediminis sp. nov., isolated from freshwater lake sediment
Author(s) -
Jianhang Qu,
Lu-Jie Zhang,
Yun-Hui Fu,
Haifeng Li
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003177
Subject(s) - 16s ribosomal rna , biology , phylogenetic tree , strain (injury) , genomic dna , microbiology and biotechnology , bacteroidetes , gene , bacteria , genus , botany , genetics , anatomy
A novel Gram-stain-negative, aerobic and rod-shaped bacterial strain, designated H-1 T , was isolated from the interfacial sediment of Taihu Lake in China and characterized by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the organism was most closely related to Rufibacter immobilis MCC P1 T and Rufibacter tibetensis 1351 T , with sequence similarities of 98.0 and 97.6 %, respectively. DNA-DNA relatedness between strain H-1 T and R. immobilis MCC P1 T and R. tibetensis 1351 T was 48.8 and 36.6 %, respectively. The major (>5 %) cellular fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 1ω5c, iso-C15 : 0, summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B) and anteiso-C15 : 0. The polar lipids comprised phosphatidylethanolamine, three unidentified aminophospholipids, an unidentified phospholipid and three unidentified lipids. The major respiratory quinone was menaquinone 7 (MK-7). Genomic DNA G+C content was 49.0 mol%. Based on its physiological, biochemical and chemotaxonomic characteristics, the strain represents a novel species of the genus Rufibacter, for which the name Rufibacter sediminis sp. nov. (type strain H-1 T =CGMCC 1.16289 T =NBRC 113030 T ) is proposed.