Open AccessJiangella endophytica sp. nov., an endophytic actinomycete isolated from the rhizome of Kaempferia elegans
Author(s) -
Nantawan Niemhom,
Chanikul Chutrakul,
Chanwit Suriyachadkun,
Sarin Tadtong,
Chitti Thawai
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003175
Subject(s) - biology , 16s ribosomal rna , strain (injury) , diamino acid , rhizome , peptidoglycan , botany , genus , microbiology and biotechnology , genomic dna , ribosomal rna , bacteria , cell wall , gene , gene sequence , genetics , anatomy
An endophytic actinobacterium, designated strain KE2-3 T , was isolated from surface-sterilised rhizome of Kaempferia elegans. The polyphasic approach was used for evaluating the taxonomic position of this strain. The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-diaminopimelic acid in the cell peptidoglycan, MK-9(H4) as the major menaquinone, iso-C16 : 0, anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes. Based on 16S rRNA gene sequence analysis, strain KE2-3 T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357 T (99.3 %) followed by Jiangella albaDSM 45237 T (99.2 %), Jiangella alkaliphilia DSM 45079 T (99.0 %), Jiangella gansuensisDSM 44835 T (98.8 %) and Jiangella mangrovi3SM4-07 T (98.6 %). However, the draft genome sequence of strain KE2-3 T exhibited low average nucleotide identity values to the reference strains (85.5-90.2 %), which were well below the 95-96 % species circumscription threshold. The DNA G+C content of genomic DNA was 72.3 mol%. With the differences of physiological, biochemical and genotypic data, strain KE2-3 T could be discriminated from its closest neighbour. Thus, strain KE2-3 T should be recognised as a novel species of genus Jiangella, for which the name Jiangellaendophytica sp. nov. is proposed. The type strain is KE2-3 T (=BCC 66359 T =NBRC 110004 T ).