Open AccessLysobacter tabacisoli sp. nov., isolated from rhizosphere soil of Nicotiana tabacum L.
Author(s) -
Min Xiao,
Xing-Kui Zhou,
Xing Chen,
Yan-Qing Duan,
Dalal Hussien M. Alkhalifah,
WanTaek Im,
Wael N. Hozzein,
Wei Chen,
WenJun Li
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003164
Subject(s) - biology , rhizosphere , nicotiana tabacum , botany , bacteria , genetics , gene
A Gram-stain-negative, aerobic, rod-shaped bacterium, designated strain C8-1 T , was isolated from the rhizosphere soil of Nicotiana tabacum L. collected from Kunming, south-west China. The cells showed oxidase-positive and catalase-positive reactions. Growth was observed at 10-40 °C, at pH 6.0-8.0 and in the presence of up to 1 % (w/v) NaCl, with optimal growth at 30 °C and pH 7.0. The predominant isoprenoid quinone was Q-8. The major fatty acids were identified as iso-C15 : 0, iso-C17 : 0 and iso-C17 : 1ω9c. The cellular polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, five unidentified phospholipids and two unidentified aminophospholipids. The genomic DNA G+C content was 70.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain C8-1 T should be assigned to the genus Lysobacter. 16S rRNA gene sequence similarity analysis showed that strain C8-1 T was closely related to Lysobacter cavernae YIM C01544 T (98.6 %), Lysobacter soli DCY21 T (97.6 %), Lysobacter panacisoli CJ29 T (97.3 %), Lysobacter firmicutimachus PB-6250 T (97.3 %), Lysobacter niastensis GH41-7 T (97.3 %) and Lysobacter gummosus KCTC 12132 T (97.1 %). DNA-DNA hybridization data indicated that the isolate may represent a novel genomic species belonging to the genus Lysobacter. Polyphasic taxonomic characteristics indicated that strain C8-1 T represents a novel species of the genus Lysobacter, for which the name Lysobacter tabacisoli sp. nov. is proposed. The type strain is C8-1 T (=KCTC 62034 T =CGMCC 1.16271 T ) .