Open AccessLutibaculum pontilimi sp. nov., isolated from a tidal mudflat and emended description of the genus Lutibaculum
Author(s) -
Soon Dong Lee
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003163
Subject(s) - biology , 16s ribosomal rna , strain (injury) , microbiology and biotechnology , glycolipid , bacteria , ribosomal rna , genus , sequence analysis , gene , zoology , genetics , biochemistry , anatomy
A novel Gram-reaction-negative bacterium, designated strain GH1-34 T , was isolated from a sample of tidal mudflat collected at the seashore of Gangwha Island, Republic of Korea. Cells of the bacterium were strictly aerobic, catalase- and oxidase-positive, motile by means of a polar flagellum and rod shaped. It was found to grow at 0-5 % (w/v) NaCl, 20-45 °C and pH 6-10. The major isoprenoid quinone was Q-10. The polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminolipid and an unidentified glycolipid. The predominant fatty acids were C18 : 1ω7c and C19 : 0cyclo ω8c. The G+C content of the DNA was 70.9 mol%. Comparative 16S rRNA gene sequence analysis revealed that strain GH1-34 T formed a tight cluster with the type strain of Lutibaculum baratangense with 98.3 % sequence similarity; levels of the 16S rRNA gene sequence similarity between the novel strain and other representatives of the order 'Rhizobiales' were <95.0 %. DNA-DNA relatedness between the organism and L. baratangense KCTC 22669 T was 33 %, Based on the results of phenotypic analysis and DNA-DNA hybridization experiments, strain GH1-34 T (=KCTC 52847 T =NBRC 113277 T ) represents a novel species of the genus Lutibaculum, for which the name Lutibaculum pontilimi sp. nov. is proposed.