Open AccessNocardia mangyaensis sp. nov., a novel actinomycete isolated from crude-oil-contaminated soil
Author(s) -
Ruiqi Yang,
Binglin Zhang,
Hongfan Sun,
Gaosen Zhang,
Shi-Weng Li,
Guangxiu Lu,
Tuo Chen,
Yunshi Li,
Yingjie Wu,
Lizhe An,
Wei Zhang,
Xiukun Wu
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003159
Subject(s) - biology , 16s ribosomal rna , microbiology and biotechnology , diaminopimelic acid , glycolipid , nocardiaceae , strain (injury) , nocardia , arabinose , nocardiosis , diamino acid , bacteria , actinomycetales , biochemistry , gene , xylose , gene sequence , streptomyces , fermentation , genetics , anatomy
A Gram-stain-positive, aerobic, non-motile and mycolic-acid-containing strain, designated Y48 T , was isolated from soil contaminated by crude oil located in the northern margin of the Qaidam Basin. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Y48 T belongs to the genus Nocardia and is closely related to N. cummidelens DSM 44490 T (99.0 % similarity), N. soli DSM 44488 T (99.0 %), N. lasii 3C-HV12 T (98.9 %), N. salmonicida NBRC 13393 T (98.6 %), N. ignorata NBRC 108230 T (98.6 %) and N. coubleae NBRC 108252 T (98.6 %). The average nucleotide identity and DNA-DNA hybridization values between strain Y48 T and the reference strains were 75.9-84.5 and 27.5-29.0 %, respectively, values that were below the thresholds for species delineation. Chemotaxonomic analysis indicated that the major fatty acids of strain Y48 T were C16 : 0, summed feature 3 (C16 : 1ω6c/C16 : 1ω7c), C18 : 1ω9c and C18 : 0 10-methyl (TBSA). The respiratory quinone was MK-8(H4, ω-cycl). The polar lipid profile was composed of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, two glycolipids and three unidentified lipids. The cell-wall hydrolysates contained meso-diaminopimelic acid, with ribose, arabinose, glucose and galactose as whole-cell sugars. A combination of 16S rRNA gene sequence analysis, and phenotypic and chemotaxonomic characterizations demonstrated that strain Y48 T represents a novel species of the genus Nocardia, for which the name Nocardia mangyaensis sp. nov. is proposed. The type strain is Y48 T (=JCM 32795 T =CGMCC 4.7494 T ).