Open AccessVallitalea okinawensis sp. nov., isolated from Okinawa Trough sediment and emended description of the genus Vallitalea
Author(s) -
Yutong Sun,
Nan Zhou,
Baojun Wang,
Xiaodong Liu,
ChengYing Jiang,
Xin Ge,
ShuangJiang Liu
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003158
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phylogenetic tree , microbiology and biotechnology , botany , ribosomal rna , glycolipid , gene , genus , genome size , genome , genetics , biochemistry , anatomy
A polyphasic study was conducted to characterize an obligately anaerobic bacterial strain, S15 T , that was isolated from Okinawa Trough sediment. Strain S15 T was Gram-stain-negative, non-motile and rod-shaped. Spores were not observed. Strain S15 T grew anaerobically at 20-35 °C (optimum at 25-30 °C) and at pH range of 6.0-8.5 (optimum at 7.5). Analysis of 16S rRNA gene sequences showed that strain S15 T was phylogenetically related to Vallitalea guaymasensis Ra1766G1 T (94.0 %) and Vallitalea pronyensis FatNI3 T (93.1 %). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and glycolipids. The predominant fatty acids of strain S15 T were iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0 and C16 : 0. The draft genome was 5.86 Mb with a DNA G+C content of 33.9 mol%. A total of 5285 genes were predicted and, of those, 4669 genes were annotated. The genome data supported the result that strain S15 T assimilated various carbon sources. On the basis of unique phenotypic, chemotaxonomic and phylogenetic comparisons, strain S15 T is proposed to represent a novel species within the genus Vallitalea, and the name Vallitaleaokinawensis sp. nov. is proposed. The type strain is S15 T =CGMCC 1.5231 T =KCTC 15675 T .