Open AccessTrinickia diaoshuihuensis sp. nov., a plant growth promoting bacterium isolated from soil
Author(s) -
Yansong Fu,
Rui Yan,
Dongli Liu,
Shuting Jiang,
Lin Cui,
Xiaowei Guo,
Xiangjing Wang,
Ji Zhang,
Wensheng Xiang
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003155
Subject(s) - biology , bacteria , plant growth , botany , microbiology and biotechnology , genetics
A novel Gram-stain-negative, aerobic, rod-shaped plant growth promoting bacterium, NEAU-SY24 T , was isolated from soil in Diaoshuihu, Heilongjiang, China. The isolate grew at temperatures 10-40 °C (optimum, 30 °C), pH 5-8 (optimum, pH 6) and in the presence of up to 1 % (w/v) NaCl, although NaCl was not required for growth. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain NEAU-SY24 T belonged to the genus Trinickia and was closely related to Trinickia dabaoshanensis NRRL B-59553 T (99.16 % similarity) and Trinickia soli DSM 18235 T (99.11 %). The average nucleotide identity values between NEAU-SY24 T and its most closely related species were 79.30-87.09 %. The in silico DNA-DNA hybridization values between NEAU-SY24 T and T. dabaoshanensis NRRL B-59553 T and T. soli DSM 18235 T were 29.30 and 24.00 %, respectively, again indicating they belong to different taxa. The genomic DNA G+C content was 63.3 mol%. The major cellular fatty acids were C17 : 0cyclo, C18 : 1ω7c, C16 : 0, summed feature 2 (comprising C14 : 0 3-OH and/or C16 : 1iso I) and C16 : 0 3-OH. The predominant respiratory quinone was Q-8 and the whole-cell sugars contained ribose, glucose and galactose. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine and two unidentified aminolipids. On the basis of morphological, physiological, biochemical and chemotaxonomic analysis, strain NEAU-SY24 T was classified as a novel species in the genus Trinickia, for which the name Trinickiadiaoshuihuensis sp. nov. is proposed. The type strain is NEAU-SY24 T (=DSM 106065 T =CCTCC AA 2018003 T ).