Open AccessDyella tabacisoli sp. nov., a bacterium isolated from an arable soil sample of Nicotiana tabacum L.
Author(s) -
Qingqing Li,
Xing Chen,
Xing-Kui Zhou,
Limin Dong,
Min Xiao,
BaoZhu Fang,
Wenjun Li,
Yan-Qing Duan
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003138
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phylogenetic tree , botany , genomic dna , genus , bacteria , microbiology and biotechnology , gene , biochemistry , genetics , anatomy
A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium, designated strain L4-6 T , was isolated from an arable soil sample of tobacco in Huize, south-western China and subjected to polyphasic taxonomic characterization. The cells showed oxidase-positive and catalase-positive reactions. Growth occurred at 20-35 °C, at pH 5.0-8.0 and with 0-2 % (w/v) NaCl, optimally at 30 °C, pH 6.0-7.0 and 0-1 % (w/v) NaCl. The major respiratory lipoquinone was ubiquinone-8. The predominant cellular fatty acids (>10.0 %) were identified as summed feature 9 (iso-C 17 : 1 ω9 c or C 16 : 0 10-methyl), iso-C 15 : 0 and iso-C 17 : 0 . The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, two unidentified aminophospholipids and five unidentified aminolipids. The genomic DNA G+C content was 60.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain L4-6 T should be affiliated to the genus Dyella and formed a clade with the most closely related organism Dyella soli JS12-10 T . 16S rRNA gene sequences similarity analysis showed that strain L4-6 T was mostly closely related to D. soli JS12-10 T (98.73 %) and Dyella lipolytica DHOB07 T (98.02 %). DNA-DNA hybridization data indicated that strain L4-6 T represented a novel genomic species belonging to the genus Dyella . The polyphasic taxonomic characteristics indicated that the strain L4-6 T represents a novel species of the genus Dyella , for which the name Dyellatabacisoli sp. nov. (type strain L4-6 T =CGMCC 1.16273 T =KCTC 62035 T ) is proposed.