Open AccessParacandidimonas caeni sp. nov., isolated from sludge
Author(s) -
Yao Li,
Yuhan Lai,
Fei Xue,
Lina Sun,
Jialian Wang
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003131
Subject(s) - biology , microbiology and biotechnology
A beige-pigmented, Gram-stain-negative, aerobic, non-motile, rod-shaped bacterium, named 24 T , was isolated from sludge of a pesticide manufacturing factory in Nantong, Jiangsu Province, China. 16S rRNA gene sequence analysis revealed that strain 24 T shared highest similarity with Parapusillimonas granuli Ch07 T (98.20 %), followed by Candidimonas nitroreducens SC-089 T (98.07 %) and Paracandidimonas soli IMT-305 T (98.03 %). Phylogenetic trees showed that strain 24 T formed a distinct clade with Paracandidimonas soli IMT-305 T . The results of DNA-DNA hybridization tests showed that reassociation values were less than 45 % with respect to these closely related type strains. Strain 24 T contained Q-8 and putrescine as the major respiratory quinone and polyamine, respectively. The main cellular fatty acids were summed feature 3 (C 16 : 1 ω7c/C 16 : 1 ω6 c ), summed feature 2 (iso-C 16 : 1 I/C 14 0 3-OH/C 12 : 0 aldehyde), summed feature 8 (C 18 : 1 ω7c/C 18 : 1 ω6c) and C 12 : 0 . The polar lipid profile included phosphatidylmethylethanolamin, phosphatidylethanolamine, phosphatidylglycerol, one unidentified phospholipid and one unidentified aminolipid. The G+C content was 56.83 mol%. Combined data from phenotypic, phylogenetic and DNA-DNA relatedness studies demonstrated that strain 24 T represents a novel species of the genus Paracandidimonas , for which the name Paracandidimonas caeni sp. nov. is proposed. The type strain is 24 T (=CCTCC AB 2018057 T =KACC 19692 T ).