Open AccessPaenibacillus flagellatus sp. nov., isolated from selenium mineral soil
Author(s) -
Xingli Dai,
Kaixiang Shi,
Xin Wang,
Jun Fan,
Rui Wang,
Shixue Zheng,
Gejiao Wang
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003125
Subject(s) - biology , 16s ribosomal rna , peptidoglycan , microbiology and biotechnology , phosphatidylglycerol , paenibacillus , strain (injury) , glycolipid , bacteria , ribosomal rna , cell wall , gene , biochemistry , phospholipid , genetics , anatomy , membrane , phosphatidylcholine
Strain DXL2 T , a Gram-stain-negative, rod-shaped, endospore-forming, motile, aerobic bacterium, was isolated from selenium mineral soil. DXL2 T had the highest 16S rRNA gene sequence similarities with those of Paenibacillus ginsengarviGsoil 139 T (96.8 %), Paenibacillushemerocallicola DLE-12 T (95.5 %) and Paenibacillus hodogayensisSG T (95.4 %). The genome size of DXL2 T was 7.24 Mb, containing 6243 predicted protein-coding genes, with a DNA G+C content of 60.2 mol%. DXL2 T contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The major cellular fatty acids were anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0. The major quinone was menaquinone 7. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two aminophospholipids, an unidentified aminolipid, phosphatidylmethylethanolamine, an unidentified glycolipid and an unidentified phospholipid. Compared with the other strains, DXL2 T had a specific phospholipid and a specific aminolipid, it hydrolyzed Tween 40 and could not assimilate potassium gluconate. On the basis of the phenotypic, chemotaxonomic and phylogenetic results, strain DXL2 T represents a novel species within the genus Paenibacillus, for which the name Paenibacillusflagellatus sp. nov. is proposed. The type strain is DXL2 T (=KCTC 33976 T =CCTCC AB 2018054 T ).